ROLE OF CYTOGENETICS IN FIRST TRIMESTER ABORTION USING PRODUCT OF CONCEPTION AND EXTENT OF SOMATIC DNA DAMAGES IN MATERNAL BLOOD

Sumitha Prabhu PS, Jeniva Baruah, Komala PT, Arun William, Aneesh P, Dinesh Roy D

Abstract


Spontaneous abortion (miscarriage) is most common during the first trimester of pregnancy. A fetal loss is occurring when the fetus has died within 8-12 weeks of gestation without heart activity has been recorded. Miscarriages affect 15% of women, primarily in the first trimester. Forty two women suffering with first trimester abortion were referred from various maternity centres of Kerala to Genetika, centre for advanced genetic studies, Trivandrum for chromosome analysis were taken as study subjects and twenty age sex match healthy women are selected as control subjects. The present study was undertaken to evaluate the role of cytogenetics in first trimester abortion using product of conception and extent of somatic DNA damages in maternal blood using Cytokinesis Block Micronuclei (CBMN) assay.
The results were computed and correlated with various other demographic, physiological, clinical and lifestyle characteristics. Regarding the karyotype of POC, 69% showed abnormal karyotype and 31% showed normal karyotype. The mean CBMN frequency of study subjects was 12.05 and control was 10.02 and showed a statistical significance. The study revealed that, the chromosomal abnormalities play an important role in first trimester pregnancy losses and these abnormalities were associated with various demographic, physiological, clinical and lifestyle characteristics. Thus, cytogenetic study should be offered to all couples especially for mother with two or more abortions. Patients who have had an unexplained pregnancy loss should be offered genetic counseling with an option for karyotype analysis for finding the role of cytogenetic in pregnancy losses. There by proper management of pregnancy losses in the future can be avoided.

 


Keywords


First Trimester Abortion, Products Of Conception, Birth Defects, Chromosome Abnormalities, Dna Damage.

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