OXIDATIVE STRESS AND DNA DAMAGES IN BAD OBSTETRIC HISTORY

Lekshmi VS, Sumitha Prabhu PS, Aneesh P, Arun William, Dinesh Roy D

Abstract


Bad obstetric history (BOH) implies previous unfavourable foetal outcome in terms of two or more consecutive spontaneous abortions, history of intrauterine foetal death, intrauterine growth retardation, stillbirth, early neonatal death and/or congenital anomalies. Twenty couples suffering from bad obstetric history were selected as study subjects. Fifteen healthy age matched couples with one or two live children were selected as control subjects for the present study. The study was undertaken to assess the effect of increased oxidative stress and DNA damages in couples experiencing BOH. Malondialdehyde (MDA) test was performed to detect the oxidative stress in patients with BOH and the extent of somatic DNA damage was quantified by Cytokinesis Block Micronuclei assay. The results of mean MDA concentration and the mean CBMN frequency showed a statistically significant difference between the study and control subjects. Subjects with increased hormone level (FSH and LH) also showed increased MDA value and mean CBMN frequency. Similar result was observed among subjects with TORCH infections. Male study subjects having the unhealthy lifestyle habits and abnormal semen parameters showed increased MDA value and mean CBMN frequency. The results were correlated with various demographic, lifestyle and clinical aspects of the patients. Modification of life style, by changing the dietary habit and sedentary life style will help to reduce the oxidative stress. Moreover, the diagnosis of chromosomal anomalies at the exact time can lead to prevention of future birth of affected babies and also pregnancy loss due to suspected genetic loss can also be reduced in the society to an extent.


Keywords


Bad obstetric history (BOH), Malondialdehyde (MDA), Recurrent miscarriage (RM)

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