OXIDATIVE STRESS AND DNA DAMAGES IN POF SUBJECTS AND ITS ASSOCIATION WITH ANTHROPOMETRIC FACTORS

Deepthi T, Anitha C, Sarala Gopalakrihnan, Dinesh Roy D

Abstract


The concept of premature ovarian failure (POF) is the cessation of ovarian function before the age of 40, associated with elevated gonadotropins
serum levels (FSH ≥ 40 UI/l) and at least 1%–3% of women of reproductive age were affected and this leads to infertility. The POF etiology is
highly disparate including genetic, autoimmune, metabolic and infectious causes, but in most cases the etiology remains as an open question. The
role of oxidative stress in the pathogenesis of POF has not studied extensively and there is no such corresponding data that states the association of
anthropometric variables and DNA damages in POF subjects. The study was taken to observe the association of anthropometric variables with
oxidative stress leading to DNA damage in POF subjects. The present study comprised of 100 subjects with POF and 100 healthy age and sex
matched control subjects. Their anthropometric measurements such as height, weight and abdominal circumference were measured and the Body
Mass Index (BMI) was calculated. The mean malondialdehyde (MDA) value was taken as the marker for oxidative stress. The DNA damages were
analyzed using Cytokinesis Block Micronuclei (CBMN) Assay. The results of the present study support the close association of anthropometric
variables in POF subjects with oxidative stress. The study clearly indicates that the study subjects have higher mean value of MDA than the control
subjects. Moreover, the DNA damage was also found to be higher among the study subjects. Thus the study explains the strong association of
anthropometric variables with oxidative stress leading to increased DNA damages. Therefore the findings of the study confirm the importance of
maintaining a healthy life along with physical activity and improved diet providing the antioxidants as well as a proper medication in order to
reduce the risk of DNA damage by lowering the oxidative stress levels in POF subjects.


Keywords


Body Mass Index, CBMN Assay, Infertility, Malondialdehyde, Micronuclei frequency, Obesity

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