SCREENING, ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF LACCASE PRODUCING BACTERIA FROM RAIGAD DISTRICT SOIL

Mr. S. N. Disale, Dr. P. P. Dixit

Abstract


Screening and isolation of Laccase producing bacteria from Raigad District soil was carried out to assess the diversity of Lignocellulose degrading
bacteria. For isolation Laccase producing bacteria nutrient medium containing guaiacol was used. Total ten isolates were isolated from soil samples
collected from different regions of Raigad district. Preliminary screening of Laccase producing bacteria was carried out by growing isolated
colonies on to the nutrient medium containing guaiacol. Green colour formation by using ABTS confirms the potential laccase producing
capability of isolates. Ten bacterial isolates showed positive results. As an outcome predominant isolates were identified as Pseudomonas spp.
Bacillus sp. The findings show that these microorganisms have significant potential for use in applications for the treatment of Lignocellulose
degradation and lignin related environment pollutants.


Keywords


Laccase, Guaiacol, Nutrient medium, Bacillus spp.

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