A STUDY ON CORRELATION BETWEEN BIOFILM PRODUCTION AND VANCOMYCIN RESISTANCE IN ENTEROCOCCAL ISOLATES OBTAINED FROM A TERTIARY CARE HOSPITAL

V G Praveena, Radhika Katragadda, Sowmya A V

Abstract


Enterococci , a normal intestinal flora in animals and humans have been identified as a significant pathogen causing infection to humans. The
following study was conducted to isolate the enterococci in various clinical samples, to demonstrate their antibiotic resistance pattern with special
reference to vancomycin and to delineate a correlation between biofilm production and expression of the vancomycin resistant genes. 200
enterococcal species isolated from various clinical samples during the study period, were subjected to antimicrobial susceptibility testing and also
assayed for their ability to form biofilms. Multiplex PCR was carried out to detect the presence of van A, van B and van C resistant genes, in the
clinical isolates. Out of the 200 enterococcal isolates, 66% were E.feacalis and 34% were E.feacium. 65% of the total enterococcal isolates were
from urine samples, followed by wound swab& pus (25%). Vancomycin resistance was found in 4% of the enterococcal isolates. Only 12% of the
total Enterococcal isolates were biofilm producers. All the vancomycin resistant isolates were strong biofilm producers (100%) and expressed only
van A resistant genes. Our study shows that enterococcus is one of the commonest uropathogen with presence of significant VRE strains,
expressing Van A resistant genes and with strong biofilm forming ability. Proper identification of VRE strains along with strict infection control
practices will help to reduce the morbidity and mortality associated with infections caused by them.


Keywords


Vancomycin resistant Enterococci, Biofilm producers, Van A resistant genes.

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