SYNTHETIC PEPTIDES AS AN EMERGING TOOL FOR THE SEROLOGICAL DIAGNOSIS OF DENGUE VIRUS INFECTION

R. Praveena, J. Asnet Mary

Abstract


Dengue fever (DF) is one of the fastest emerging arboviral diseases and has become a major public health concern across the world. It is caused by dengue virus, belonging to genus flavivirus. It causes an acute, systemic febrile illness. Specific treatments for Dengue viruses are not available so far. It is imperative to diagnose the dengue virus at the early stages of infection to curb mortality. The basic laboratory diagnosis methods detect antigen or antibodies. In this study, peptide antigen was used to diagnose the virus Indirect ELISA. Three synthetic peptides, Peptide-1-LEHGSCVTTMAKDKPTL, Peptide-2-DRGWGNGCGLFG and Peptide-3-CGQGKAHNGRLITANP were used for the serological diagnosis. Accuracy, sensitivity and specificity were calculated for their potential to differentiate positive and negative samples. All statistical analyses were performed using MedCalc software. The results showed that Peptide-1, Peptide-2 and Peptide-3 have high sensitivity of 90%, 96.7%, 95% respectively. The specificity of the peptides is also 70%, 75%, 70% for Peptide-1, Peptide-2, Peptide-3 respectively. The results suggested that the peptide based diagnosis is an alternative method for the early diagnosis of Dengue virus.


Keywords


Dengue virus, Peptides, Diagnosis, Specificity, ELISA, Sensitivity

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