MTT BIO ASSAY OF LACTOFERRICIN AND TACHYPLESIN ON THREE CANCER CELL LINES

N. Trivikram, Dr. S. P. Sreedharbhattar

Abstract


In the advanced Research investigation, anti-cancer agents are an exciting and important part of medicinal chemistry due to its prevalence in the population. In our present study, Tachyplesin and lactoferrin are antimicrobial peptides isolated from cow, buffalo and sheep has been shown to kill cancer cells. So we focused to evaluate the peptide as an antibiotic as preliminary work to provoke and further analyses by testing the effects against cancer cells. In the study, before working on cancer lines, preliminary testing has done by MTT assay. An MTT assay was characterized to count percent of cell viability with relation to increasing concentrations of peptides. IC50 of lactoferricin towards HTB9, MFC-7 and PC-3cell lines were calculated at 784.1ug/ml, 697.1 µg/ml and841.2 µg/ml concentrations of anticancer peptide respectively. IC50 was calculated for the activity of Tachyplesin on all cell lines and recorded to be 452.9 µg/ml, 203.4 µg/ml and 281.0 µg/ml for HTB9, MFC-7 and PC-3 cell lines respectively. Results proved and gave assurance to have viability control effects itself.


Keywords


Lactoferrin, Tachyplesin, cancer cell lines -breast cancer cells (MFC-7), Prostate cancer cells (PC-3) and Renal cancer cells (HTB9

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