CHROMOGENIC SPRAY FOR DETECTION AND IDENTIFICATION OF PARAPHENYLENEDIAMINE FROM BIOLOGICAL MATERIAL

Charansing B. Ghoti, Keshav S. Kapgate, Shivangi S. Apte, Vijay J. Thakre

Abstract


Thin layer chromatography (TLC) is a simple, rapid and reliable technique usually used in forensic
science laboratory for detection of poison in biological material. It can separate many complex mixtures
in a short period of time. In this study an effort has been taken to determine Paraphenylenediamine by using Thin Layer
chromatography. A specic chromogenic reagent Ninhydrin in ethanol has been used for the detection of
Paraphenylenediamine with Chloroform: Acetone (1:1) as a solvent system for the separation.


Keywords


Paraphenylenediamine, Ninhydrin, Hair Dyes, Forensic Science, Biological Material.

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