STUDY OF SERUM PARAOXONASE 1 LEVELS AND PARAOXONASE 1 GENE (192A/G) POLYMORPHISM IN TYPE 2 DIABETES MELLITUS.

Swati Soni, Ritu Singh, Amita Yadav, Anupam Parkash

Abstract


Paraoxonase1 is a HDL associate antioxidant enzyme helping in evaluating oxidative stress status and
complications in diabetes mellitus. Paraoxonase gene polymorphism inuence Paraoxonase 1 levels
and may be the molecular basis for inter individual variability seen in Diabetes Mellitus. Hence, this study was taken up to
analyze the concentration of serum Paraoxonase 1 levels and Paraoxonase 1 gene (192A/G) polymorphism in type 2
diabetes.The study included 40 patients (≥ 30 years) with diabetes mellitus and 40 ages and sex matched healthy controls.
Paraoxonase 1 level were determined ELISA and PCR assay targeting Paraoxonase 192(A/G) gene was done. Restriction
enzyme Alw1 is used to digest PCR product.There was a signicant reduction in Paraoxonase1 levels (p value =0.005) among
the Type 2 D M patients compared to healthy controls along with higher Paraoxonase levels in AA genotype (251.47±299.07
pg/ml) as compared to AG genotype (120.73±17.88pg/ml) with p value = 0.024 showing the G variant to be more prone to
oxidative damage caused by hyperglycemia thereby lowering the Paraoxonase 1 levels. The results were analyzed using 20th
version of SPSS.T2 DM patients have increased oxidative stress as indicated by decreased levels of enzyme Paraoxonase 1.
This reduction in PON1 activity has been suggested as a reason for the increased risk of cardiac disease in these patients. In
this study the G variant is more prone to oxidative damage caused by hyperglycemia thereby lowering the Paraoxonase 1
levels.


Keywords


Paraoxonase 1, Paraoxonase 1 Gene (192A/G) Polymorphism, Type 2 Diabetes Mellitus.

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